hutsmc (ATCC)

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ATCC hutsmc

(A) Western blots showing expression of mesenchymal markers (ZEB1 and Slug) and NFI TFs in untransformed human uterine smooth muscle cells <t>(HUtSMC)</t> versus 3 leiomyosarcoma cell lines <t>(Leio-012,</t> <t>SK-LMS-01,</t> and SK-UT-1B). (B) Western blots showing expression of smooth muscle markers (smooth muscle actin or SMA and MLK1) and AP-1 TFs (FOSL2, BACH2) in the aforementioned cell lines. (C-D) Relative mRNA expression from qRT-PCR showing depletion of the indicated TFs with shRNAs in SK-UT-1B (mimics MES) and Leio-012 (mimics SMC). (E) Relative proliferation of SK-UT-1B cells treated with shRNAs targeting the indicated NFI TFs. (F) Relative proliferation of Leio-012 cells treated with shRNAs targeting the indicated AP-1 TFs. (G) Representative images of invasion assay. (H) Quantification of invasion assay. (I-J) Tumor volume over time following xenografting into immunodeficient nude mice. Timepoints represent means ± SEM for 5 mice. *P value < 0.05; **P value < 0.01.

Hutsmc, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sk+ut/SK-UT-1B/bio_rxiv__64898__2026__05__22__726988-266-0-9
Average 94 stars, based on 63 article reviews

hutsmc - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "Spatially-Resolved Multiomic Atlas of Leiomyosarcoma Identifies Two Clinically Relevant Epigenetically-Driven Cell States"

Article Title: Spatially-Resolved Multiomic Atlas of Leiomyosarcoma Identifies Two Clinically Relevant Epigenetically-Driven Cell States

Journal: bioRxiv

doi: 10.64898/2026.05.22.726988

(A) Western blots showing expression of mesenchymal markers (ZEB1 and Slug) and NFI TFs in untransformed human uterine smooth muscle cells (HUtSMC) versus 3 leiomyosarcoma cell lines (Leio-012, SK-LMS-01, and SK-UT-1B). (B) Western blots showing expression of smooth muscle markers (smooth muscle actin or SMA and MLK1) and AP-1 TFs (FOSL2, BACH2) in the aforementioned cell lines. (C-D) Relative mRNA expression from qRT-PCR showing depletion of the indicated TFs with shRNAs in SK-UT-1B (mimics MES) and Leio-012 (mimics SMC). (E) Relative proliferation of SK-UT-1B cells treated with shRNAs targeting the indicated NFI TFs. (F) Relative proliferation of Leio-012 cells treated with shRNAs targeting the indicated AP-1 TFs. (G) Representative images of invasion assay. (H) Quantification of invasion assay. (I-J) Tumor volume over time following xenografting into immunodeficient nude mice. Timepoints represent means ± SEM for 5 mice. *P value < 0.05; **P value < 0.01.

Figure Legend Snippet: (A) Western blots showing expression of mesenchymal markers (ZEB1 and Slug) and NFI TFs in untransformed human uterine smooth muscle cells (HUtSMC) versus 3 leiomyosarcoma cell lines (Leio-012, SK-LMS-01, and SK-UT-1B). (B) Western blots showing expression of smooth muscle markers (smooth muscle actin or SMA and MLK1) and AP-1 TFs (FOSL2, BACH2) in the aforementioned cell lines. (C-D) Relative mRNA expression from qRT-PCR showing depletion of the indicated TFs with shRNAs in SK-UT-1B (mimics MES) and Leio-012 (mimics SMC). (E) Relative proliferation of SK-UT-1B cells treated with shRNAs targeting the indicated NFI TFs. (F) Relative proliferation of Leio-012 cells treated with shRNAs targeting the indicated AP-1 TFs. (G) Representative images of invasion assay. (H) Quantification of invasion assay. (I-J) Tumor volume over time following xenografting into immunodeficient nude mice. Timepoints represent means ± SEM for 5 mice. *P value < 0.05; **P value < 0.01.

Techniques Used: Western Blot, Expressing, Quantitative RT-PCR, Invasion Assay

other:

Article Title: In Vitro Assessment of the Expression and T Cell Immunogenicity of the Tumor-Associated Antigens BORIS, MUC1, hTERT, MAGE-A3 and Sp17 in Uterine Cancer
Article Snippet: The commercial cell lines SKOV-3, OVCAR-3, RL-95-2, HEC-1 and SK-UT-1 were obtained from ATCC (Manassas, VA, USA).

Article Title: Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity
Article Snippet: SK-UT-1 human uterine leiomyosarcoma cells, A431 human epidemoid carcinoma cells, HT29 human colorectal adenocarcinoma cells and HCT116 human colorectal carcinoma cells were purchased from ATCC.

Article Title: Could let-7f , miR-10b , miR-34a , miR-181b , and miR-181d Be Useful Tools as a Target Therapy for Uterine Leiomyosarcoma?
Article Snippet: PCS-460-011 (myometrium), THESCs–CRL-4003 (LM), and SK-UT-1–HTB-114 (LMS) cells obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) were grown in specific mediums and conditions as previously described [ ].

Article Title: Profiling and Functional Analyses of MicroRNAs and Their Target Gene Products in Human Uterine Leiomyomas
Article Snippet: Two uterine leiomyosarcoma cell lines SK-LMS-1 and SK-UT-1 were obtained from ATCC.

In Vitro:

Article Title: Cyclooxygenase inhibitors block uterine tumorigenesis in HMGA1a transgenic mice and human xenografts
Article Snippet: .. Cell lines and in vitro studies Human uterine cancer cell lines MES-SA and SK-UT-1 obtained from ATCC were grown as recommended. ..

Modification:

Article Title: TG-Interacting Factor Expression in Leiomyoma Compared to Myometrium
Article Snippet: .. SK-UT-1 human uterine leiomyosarcoma cells (ATCC, Manassas, VA) were maintained in high glucose Dulbecco's modified Eagle's medium (DMEM), supplemented with 10% fetalbovine serum, and 1% penicillin/streptomycin at 37°C with 5% CO 2 . ..

Cell Culture:

Article Title: Curcumin targets the AKT-mTOR pathway for uterine leiomyosarcoma tumor growth suppression.
Article Snippet: Background Uterine leiomyosarcomas generally do not respond well to standard chemotherapy.. We previously demonstrated that curcumin, the active ingredient derived from the herb Curcuma longa, inhibits uterine leiomyosarcoma cells in vitro via the inhibition of the AKT– mammalian target of rapamycin (mTOR) pathway.. As a preclinical investigation, we performed an in vivo study using female nude mice to confirm the therapeutic potential of curcumin against uterine leiomyosarcoma.

Review